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根据多房棘球绦虫emY162基因序列设计引物,利用PCR以细粒棘球绦虫原头蚴和成虫的基因组DNA和cDNA为模板扩增egG1Y162基因,构建PUCm-T/egG1Y162和PUCm-T/egG1Y162cDNA重组质粒,经PCR、酶切及测序后,进行序列分析。细粒棘球绦虫2个不同发育阶段均克隆出egG1Y162基因,从基因组DNA和cDNA克隆得到的片段长度分别为1680bp和459bp,登录号分别为AB458258和AB458259。
According to the sequence of emY162 gene of Echinococcus multilocularis, primers were designed to amplify egG1Y162 gene by PCR using genomic DNA and cDNA of protoscolex and adult worms of Echinococcus granulosus, and to construct PUCm-T / egG1Y162 and PUCm-T / egG1Y162 cDNA recombinants Plasmids were sequenced after PCR, digestion and sequencing. EgG1Y162 gene was cloned from E.granulosus in two different developmental stages. The fragments obtained from genomic DNA and cDNA clones were 1680bp and 459bp, respectively. The accession numbers were AB458258 and AB458259, respectively.