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目的:从生物热力学角度,探讨黄连不同提取部分抑菌作用差异,为黄连活性成分的研究提供新思路和理论依据。方法:采用微量量热法,在LB培养基,37℃条件下,接种量为1×10~5个/mL无菌条件下,建立大肠埃希菌、金黄色葡萄球菌和志贺氏痢疾杆菌的生物热动力学方法。利用该方法,绘制了3种菌分别在黄连水提物、生物碱部分和非生物碱部分作用下的生长热谱图(P-t图),得到相应的生物热动力学参数(主要包括生长速率常数k,细菌生长抑制率I,半数药物浓度抑制率IC_(50),最大产热功率P_(max)和细菌整达到P_(max)的时间t_(max)),综合分析不同提取部分对3种菌生长代谢作用差异。结果:黄连水提物和生物碱部分对3种菌生长有较强抑制作用,抑制顺序均为生物碱部分>水提物,而黄连非生物碱部分对3种菌生长不产生抑制作用,甚至有助菌生长的作用。同一提取部分对不同菌抑制作用顺序均为金黄色葡萄球菌>志贺氏痢疾杆菌>大肠埃希菌。结论:通过微量量热法实验结果分析,黄连的活性成分为生物碱部分,对金黄色葡萄球菌的生长抑制能力最强。
OBJECTIVE: To investigate the differences of bacteriostasis between different parts of Coptis chinensis from the point of view of biothermology, and to provide new ideas and theoretical basis for the study of active ingredients of Coptis chinensis. Methods: Micro-calorimetry was used to establish Escherichia coli, Staphylococcus aureus and Shigella dysenteriae under the sterile condition of LB medium, 37 ℃ and inoculation amount of 1 × 10-5 / mL Bio-thermokinetic method. Using this method, the growth thermograms (Pt maps) of the three kinds of bacteria under the action of the water extract of Coptidis Rhizome, the alkaloid fraction and the non-alkaloid fraction were plotted, and the corresponding thermokinetic parameters (mainly including the growth rate constant k, bacterial growth inhibition rate I, IC 50 of the half drug concentration, the maximum heat production power P max and the time t max for the bacteria to reach P max, the effects of different extracts on three Differences in growth and metabolism of bacteria. Results: The water extract of Rhizoma coptidis and alkaloids partially inhibited the growth of the three kinds of bacteria. The order of inhibition was alkaloid part> water extract, while the non-alkaloid part of Rhizoma Coptidis did not inhibit the growth of the three kinds of bacteria. Help bacteria growth. The same extraction part of different bacteria inhibitory sequence were Staphylococcus aureus> Shigella dysenteriae> Escherichia coli. Conclusion: According to the results of microcalorimetry experiment, the active ingredient of Coptis chinensis is part of alkaloids, which has the strongest inhibitory effect on the growth of Staphylococcus aureus.