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目的通过检测Axl、Gas6在甲状腺乳头状癌(PTC)中的表达,分析和探讨两者在甲状腺乳头状癌发生、发展中的作用及相关性。方法采用免疫组织化学法、Western blotting及Real-time PCR法检测Axl、Gas6在甲状腺乳头状癌中的表达并分析两者的变化与临床病理特征的关系。结果与结节性甲状腺肿(NG)及瘤旁正常组织相比,甲状腺乳头状癌组织中Axl、Gas6的mRNA及蛋白表达水平明显升高(P<0.05),且蛋白表达量与淋巴结转移、临床病理分期有关,与患者年龄、性别、肿瘤大小无关。结论 Axl和Gas6两者在PTC中的表达量高于结节性甲状腺肿。在PTC中Axl、Gas6的高表达与肿瘤的淋巴结转移和临床病理分期呈正相关,两者在PTC的发生、发展、转移的过程中起重要的作用。
Objective To investigate the role of Axl and Gas6 in the pathogenesis and progression of papillary thyroid carcinoma by detecting the expression of Axl and Gas6 in thyroid papillary carcinoma (PTC). Methods Immunohistochemistry, Western blotting and Real-time PCR were used to detect the expression of Axl and Gas6 in papillary thyroid carcinoma and their relationship with clinicopathological features. Results The mRNA and protein expressions of Ax1 and Gas6 in thyroid papillary carcinoma were significantly higher than those in nodular goiter (NG) and adjacent normal tissues (P <0.05), and the protein expression was correlated with lymph node metastasis, Clinicopathological stage, with the patient’s age, gender, tumor size has nothing to do. Conclusion The expression of Axl and Gas6 in PTC is higher than that in nodular goiter. The high expression of Axl and Gas6 in PTC is positively correlated with lymph node metastasis and clinicopathological stage of PTC, both of which play an important role in the occurrence, development and metastasis of PTC.