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目的研究特异性细胞毒Ｔ淋巴细胞（ｃｙｔｏｔｏｘｉｃＴｌｙｍｐｈｏｃｙｔｅ，ＣＴＬ）杀伤胃癌细胞ＢＧＣ８２３的超微结构变化。方法用２２例患者的胃腺癌细胞反复冻融后的滤液，抗ＣＤ３单克隆抗体、白细胞介素２（ＩＬ２）与患者自体外周血淋巴细胞共同培养，体外诱导ＣＴＬ，然后将ＣＴＬ与ＢＧＣ８２３共同孵育，用电子显微镜观察ＣＴＬ作用于ＢＧＣ８２３后的超微结构改变。结果电镜观察可见ＣＴＬ包围并破坏靶细胞，靶细胞凋亡的形态表现为异染色质浓缩呈特征性半月形帽状核，还可见靶细胞溶解坏死。结论特异性ＣＴＬ可使靶细胞凋亡，溶解而导致其死亡
Objective To study the ultrastructural changes of specific cytotoxic T lymphocytes (CTL) in killing gastric cancer cells BGC823. Methods Twenty-two patients with gastric adenocarcinoma cells were repeatedly frozen and thawed. Anti-CD3 monoclonal antibody, interleukin-2 (IL-2) was co-cultured with autologous peripheral blood lymphocytes, and CTLs were induced in vitro. The BGC-823 was incubated together and electron microscopy was used to observe the ultrastructural changes after the CTLs acted on BGC-823. RESULTS: The electron microscopy showed that CTLs surrounded and destroyed the target cells. The morphology of the target cells showed a heterogeneous half-moon cap-like nucleus with heterochromatin concentration, and the target cells also showed lysis and necrosis. Conclusion The specific CTL can cause apoptosis in target cells, which leads to its death.