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目的:研究在医院门急诊环境下输液配置及存放过程中的微生物污染情况及影响因素。方法:在A、B、C三家医院的门急诊环境下按无菌操作法配置静脉输液各400份,操作时分别穿刺1、3、6、9次各100份,配置完成后分别放置0、2、4、6、8 h各20份,按照《中国药典》2010版附录ⅪH无菌检查法项下规定的薄膜过滤法进行无菌检查。结果:三家医院培养的微生物阳性结果共147份(12.25%,147/1 200)。A、B、C三家医院的阳性结果分别58例(14.50%)、47例(11.75%)、42例(10.50%),比较差异无统计学意义(P>0.05)。穿刺次数是影响输液微生物污染的因素,不同放置时间段内均可发生微生物污染。结论:医院门急诊环境下配置和放置输液存在一定微生物污染风险,尤其在儿童医院,输液配置应在净化环境中严格按无菌操作规程进行,配置完成的输液应尽快使用。
Objective: To study the microbial contamination and its influencing factors in the configuration and storage of transfusion in hospital emergency department. Methods: 400 cases of intravenous fluids were dispensed by the aseptic technique in the outpatient and emergency departments of A, B and C hospitals respectively. Each operation was punctured with 100, 1, 3, 6 and 9 times respectively. After configuration, 2, 4, 6, 8 h each 20, in accordance with the “Chinese Pharmacopoeia” 2010 edition Appendix Ⅺ H sterile test under the provisions of the membrane filtration method for sterile inspection. Results: A total of 147 positive samples (12.25%, 147/1 200) were obtained from the three hospitals. The positive results of A, B and C were 58 cases (14.50%), 47 cases (11.75%) and 42 cases (10.50%) respectively, with no significant difference (P> 0.05). The number of punctures is a factor that affects the microbial contamination of transfusion fluids. Microbial contamination can occur during different periods of placement. Conclusion: There is a certain risk of microbial contamination in the emergency and emergency department of hospitals, especially in children’s hospitals. The infusion should be performed strictly according to the aseptic procedure in the purification environment. The infusion should be used as soon as possible.