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本实验发现正常骨髓细胞经不同温度（３７℃～４３℃）、不同时间（３０ｍｉｎ、６０ｍｉｎ和９０ｍｉｎ）其细胞形态和细胞化学染色均无明显变化。而急性非淋巴细胞白血病（ＡＮＬＬ）患者的骨髓白血病细胞随温度增高退化细胞和核溶解细胞增多。正常人骨髓粒单祖细胞（ＣＦＵ－ＧＭ）生长仅在加热４３℃才明显减少，而白血病祖细胞（ＣＦＵ－Ｌ）的生长随温度升高呈指数性下降。证明ＣＰＵ－Ｌ对高热的敏感性高于ＣＰＵ－ＧＭ，高热能选择性杀伤白血病祖细胞。４２℃６０ｍｉｎ和４３℃３０ｍｉｎ能尽可能多杀伤ＣＦＵ－Ｌ，而保留较多ＣＦＵ－ＧＭ，不影响正常骨髓造血重建，是清除骨髓ＣＦＵ－Ｌ的最适合温度。但即使加热至４３℃９０ｍｉｎ仍有少量的白血病祖细胞存在，可造成复发，最好与其他方法联合净化骨髓ＣＦＵ－Ｌ，以利自体骨髓移植（ＡＢＭＴ）持久成功。
The experiment found that the normal bone marrow cells by different temperatures (37 ℃ ~ 43 ℃), at different times (30min, 60min and 90min) cell morphology and cytochemical staining showed no significant change. However, the number of myeloid leukemia cells in acute non-lymphocytic leukemia (ANLL) patients increased with the increase of temperature. Normal human myeloid granulocyte progenitor cells (CFU-GM) growth only significantly reduced heating 43 ℃, while the growth of leukemia progenitor cells (CFU-L) exponential decline with increasing temperature. Prove that CPU-L is more sensitive to hyperthermia than CPU-GM, and high-thermal energy selectively kill leukemia progenitor cells. CFU-L could kill as much as possible at 42 ℃ for 60min and 43 ℃ for 30min, while retaining more CFU-GM did not affect the normal bone marrow hematopoietic reconstitution, which was the most suitable temperature for clearing bone marrow CFU-L. However, even a small amount of leukemia progenitor cells still exist after being heated to 43 ℃ for 90 minutes, which may cause relapse. It is best to combine with other methods to purify the bone marrow CFU-L for the long-term success of autologous bone marrow transplantation (ABMT).