白细胞介素-1受体拮抗剂对体外培养的大鼠大脑皮层神经细胞缺氧性损伤的影响及其基因转染治疗

• 作 者: 梁卫兰 母得志 张国荣 吴希如
• 机 构: 北京医科大学第一医院儿科
• 出 处: 北京医科大学学报
• 发表日期: 1999年03期
• 关键词: 皮层神经细胞 缺氧性损伤 体外培养 受体拮抗剂 基因转染 转染基因疗法 大鼠 细胞凋亡 迟发性损伤 药物作用

目的：观察ＩＬ１的受体拮抗剂（ＩＬ１ｒａ）对缺氧性神经细胞的影响，探讨ＩＬ１在神经细胞缺氧损伤中的作用以及基因转染治疗的可能性。方法：（１）以缺陷型ＨＳＶ为载体构建的ｐＨＳＶＩＬ１ｒａ转染体外培养的神经细胞为实验组，以ｐＨＳＶＬａｃＺ转染的体外培养的神经细胞为对照，进行体外缺氧实验。以神经细胞的３ＨＧＡＢＡ和３ＨＧｌｕ摄取能力及细胞凋亡情况为判定损伤程度的指标。结果：转染ｐＨＳＶＩＬ１ｒａ的神经细胞缺氧后，其３ＨＧＡＢＡ和３ＨＧｌｕ的摄取较转染ｐＨＳＶＬａｃＺ的神经细胞有所增高，神经细胞凋亡的程度少于对照。结论：ｐＨＳＶＩＬ１ｒａ转染可减轻缺氧引起的神经细胞急性损伤和迟发性损伤。

Objective: To observe the effect of IL1 receptor antagonist (IL1ra) on hypoxic neurons and to explore the role of IL1 in hypoxic injury of nerve cells and the possibility of gene transfection therapy. Methods: (1) In vitro culture of nerve cells transfected with pHSVIL1ra constructed by defective HSV vector was used as experimental group. In vitro hypoxia experiments were carried out in vitro using pHSVLacZ transfected neural cells as control. 3H-GABA neurons and 3H Glu uptake capacity and apoptosis as indicators of the degree of injury. Results: After hypoxia transfected neurons in pHSVIL1ra cells, the uptake of 3HGABA and 3HGlu in neurons was higher than that in pHSVLacZ transfected cells, and the degree of neuronal apoptosis was less than that in the control. CONCLUSIONS: Transfection with pHSVIL1ra can reduce acute and delayed neuronal injury caused by hypoxia.