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目的:研究长链非编码RNA NEAT1在肾癌组织及其癌旁组织中的表达水平差异,分析其表达水平与患者临床特征之间的关系,并探究其有何相对应的临床意义。方法:提取35对标本的总RNA,经逆转录、实时荧光定量PCR(qRT-PCR)来检测NEAT1的表达量,并使用统计学分析NEAT1的表达差异与其临床特征之间的具体相关性。结果:NEAT1在30例(85.71%))肾癌标本中表达下调,统计学分析表明NEAT1在肾癌组织表达明显下调(P<0.05)。NEAT1在肾癌中表达量与患者的性别、年龄、癌组织类型、TNM分期、AJCC分期无明显相关性(P>0.05)。结论:NEAT1在肾癌组织中表达明显下降,提示其可能与肾癌的发生发展有一定关系。
OBJECTIVE: To study the expression of long-chain non-coding RNA NEAT1 in renal cell carcinoma and its adjacent tissues, and to analyze the relationship between the expression of NEAT1 and clinicopathological characteristics and to explore its clinical significance. Methods: Total RNA was extracted from 35 pairs of specimens. The expression of NEAT1 was detected by reverse transcription and real-time quantitative PCR (qRT-PCR). The correlation between NEAT1 expression and clinical features was analyzed by statistical analysis. Results: NEAT1 was down-regulated in 30 (85.71%) renal cell carcinoma specimens. Statistical analysis showed that NEAT1 was significantly downregulated in renal cell carcinoma (P <0.05). There was no significant correlation between the expression of NEAT1 and the gender, age, type of cancer, TNM stage and AJCC stage in renal cell carcinoma (P> 0.05). Conclusion: NEAT1 expression in renal cell carcinoma decreased significantly, suggesting that it may have a certain relationship with the occurrence and development of renal cell carcinoma.