论文部分内容阅读
目的利用模式生物—秀丽隐杆线虫探讨二氧化钛(TiO2)纳米颗粒的毒性作用,为建立线虫作为毒理学评价模型提供试验依据。方法采用5,35,300 nm等3种粒径的二氧化钛对秀丽隐杆线虫以0.1,1.0,10.0 g/L等3个浓度染毒24 h,应用世代时间、后代数目、头部摆动频率和身体弯曲频率及体长等生殖、运动和发育的相关指标对纳米二氧化钛毒性作用进行综合评价。结果与对照组比较,暴露于5 nm TiO2的线虫在中高浓度暴露组均出现生殖、运动和发育等多系统的异常(P<0.05);而暴露于35 nm TiO2的线虫仅在高浓度暴露组出现生殖、运动等指标的异常(P<0.05);暴露于300 nmTiO2时高浓度暴露组的线虫出现身体弯曲频率降低(P<0.05),其他浓度组未出现差异有统计学意义的改变。结论纳米TiO2暴露可影响秀丽隐杆线虫运动、生殖和发育等多种系统,并与粒径和暴露浓度有关。
Objective To explore the toxicity of titanium dioxide (TiO 2) nanoparticles by using the model organism Caenorhabditis elegans to provide a basis for establishing a nematode as a toxicology evaluation model. Methods Three different concentrations of titanium dioxide (5,35 and 300 nm) were used to treat C. elegans at three concentrations of 0.1, 1.0 and 10.0 g / L for 24 h. The generation time, the number of offspring, the frequency of head oscillation and body flexion Frequency and body length and other reproductive, exercise and development related indicators of toxic effects of nano-titanium dioxide comprehensive evaluation. Results Compared with the control group, the nematodes exposed to 5 nm TiO2 exhibited abnormalities in multiple systems such as reproduction, movement and development (P <0.05). However, nematodes exposed to 35 nm TiO2 only exposed to high concentration of exposure (P <0.05). When exposed to 300 nm TiO 2, the frequency of body bending of the nematodes exposed to high concentrations of exposure decreased (P <0.05), and no significant difference was observed in other concentrations. Conclusion Nano-TiO2 exposure can affect many systems of movement, reproduction and development of C. elegans, and is related to the particle size and exposure concentration.