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目的:观察肺炎支原体(MP)诱导A549细胞产生肿瘤坏死因子-α(TNF-α)和白细胞介素-8(IL-8)的相关性,为MP肺炎的临床诊治提供依据。方法:体外培养A549细胞和MP,用感染复数为1:20的MP加入到A549细胞中共同孵育,采用双抗体夹心ELISA法检测不同刺激时间的MP作用A549细胞后分泌TNF-α和IL-8的含量。结果:MP能显著诱导A549细胞分泌TNF-α和IL-8(均P<0.01),经相关回归分析,其产生的水平与刺激时间具有依赖性(r=0.821,0.911,P<0.01),且二者之间具有量的正相关(r=0.942,P<0.01)。结论:MP诱导A549细胞产生的炎性细胞因子TNF-α和IL-8具有量的正相关性。
Objective: To observe the correlation between the production of tumor necrosis factor-α (TNF-α) and interleukin-8 (IL-8) in A549 cells induced by Mycoplasma pneumoniae and provide basis for the clinical diagnosis and treatment of MP pneumonia. Methods: A549 cells and MP were cultured in vitro. A549 cells were incubated with MP with a multiplicity of infection of 1:20 and then co-incubated with A549 cells. The effects of MP stimulation on TNF-α and IL-8 Content. Results: MP could significantly induce the secretion of TNF-α and IL-8 in A549 cells (all P <0.01). The level of TNF-α and IL-8 in A549 cells was dependent on the time of stimulation (r = 0.821,0.911, P <0.01) There was a positive correlation between them (r = 0.942, P <0.01). CONCLUSION: MP induces a positive correlation between the levels of inflammatory cytokines TNF-α and IL-8 in A549 cells.