四氯苯醌荷移反应分光光度法测定洛美沙星

• 作 者: 开小明
• 机 构: 安庆师范学院化学化工学院,
• 出 处: 光谱实验室
• 发表日期: 2012年06期
• 关键词: 洛美沙星 荷移反应 四氯苯醌 分光光度法

研究了在pH6.86磷酸标准缓冲溶液中,电子给体洛美沙星与电子受体四氯苯醌之间的荷移反应产物的紫外光谱性质。用摩尔比法和平衡移动法测定络合物的组成比为1:1。洛美沙星的浓度在0—17mg/L范围内符合比耳定律,r=0.9999。在测定波长336nm处,络合物的表观摩尔吸光系数为1.22×104L·mol-1·cm-1。方法用于洛美沙星胶囊的测定,其回收率为99.1%—102.3%,6次测量相对标准偏差为1.5%—2.1%。

The UV spectral properties of the charge-transfer reaction product between the electron donor lomefloxacin and the electron acceptor choloroquinone were studied in pH6.86 phosphate standard buffer solution. The composition ratio of complex was 1: 1 by molar ratio method and equilibrium shift method. Lomefloxacin concentration in the range of 0-17mg / L Beer’s law, r = 0.9999. The apparent molar absorptivity of complex was 1.22 × 104 L · mol-1 · cm-1 at the wavelength of 336 nm. The method was applied to the determination of lomefloxacin capsules with the recoveries of 99.1% -102.3% and the relative standard deviations of 6 measurements of 1.5% -2.1%.