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用不同浓度的秋水仙素与２％二甲基亚砜的复合溶液处理苹果栽培品种嗄拉和千秋的试管苗离体叶片。结果表明，以０．５％秋水仙素溶液处理４天的效果最佳，诱变频率达５６１％。诱变后获得的四倍体植株在形态学和细胞学上均发生了明显变异，与对照相比，变异株茎矮、粗壮、节间短，叶色浓厚，单位面积气孔数目减少，气孔体积增大，染色体数目加倍，２ｎ＝４ｘ＝６８（对照为２ｎ＝２ｘ＝３４），核仁数目增加。梢端组织学切片证实，获得的同质四倍体频率为８６％。
Different concentrations of colchicine and 2% dimethylsulfoxide complex solution of apple cultivars Pira and Thousand autumn vitro leaves of in vitro plantlets. The results showed that 0.5% colchicine solution for 4 days the best effect, the mutation frequency of 56 1%. Compared with the control, the tetraploid plants obtained after mutagenesis had obvious morphological and cytological variations. Compared with the control, the tetraploid plants were short stalks, stout, short internodes, thick leaves, reduced number of stomata per unit area, Increased, doubling the number of chromosomes, 2n = 4x = 68 (control 2n = 2x = 34), the number of nucleolus increased. The tip histological sections confirmed that the homogenous tetraploid obtained had a frequency of 86%.