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本文结合制定国家标准探讨了游泳池水细菌总数的测定所用培养基的成分，培养的温度、时间和ＴＴＣ对菌落计数的影响；探讨了游泳池水大肠菌群测定所用培养基的选择性和特异性以及保湿剂对大肠菌群计数的影响。试验结果证实：ＩＳＯ国际标准方法所用培养基活菌计数的检出率高于我国ＧＢ５７５０—８５２４％，２２℃培养７２ｈ检出率最高，是３７℃培养２４ｈ的１．７倍。ＩＳＯ９３０８—２滤膜法测定大肠菌群所用培养基阴性菌和阳性菌颜色分明易于辨认，分离培养基上阴性菌为绿色，阳性菌为黄色。保湿剂在ＩＳＯ规定的剂量范围内加与不加对大肠菌群计数无影响。
In this paper, the composition of culture medium, the temperature and time of culture, and the effect of TTC on the colony counting were discussed. The selectivity and specificity of culture medium used in determination of coliform bacteria in swimming pool were discussed. Effect of humectants on coliform counts. The test results confirm that the detection rate of viable count in culture medium used by ISO international standard method is higher than that of GB5750-8524% in China, and the highest detection rate at 72 ℃ for 72h is 1.7 times higher than that at 37 ℃ for 24h. The medium for determination of coliforms by the ISO9308-2 filter method was negative for the culture medium and for the positive bacteria. The color of the culture medium was clear and the negative bacteria in the culture medium was green while the positive bacteria were yellow. Humectants have no effect on colony counts with and without the addition of ISO in the dosage range specified.