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目的 系统评价聚合酶链反应-单链构象多态性(PCR-SSCP)对结核分支杆菌rpoB基因突变耐利福平的诊断价值.方法 计算机检索PubMed、Web of Science、The Cochrane Library(2014年第2期)、CBM、VIP和WanFang Data,查找PCR-SSCP与金标准(常规体外药敏试验结果)比较检测结核分支杆菌rpoB基因突变的诊断性研究,检索时限均为从建库至2014年1月.由2位评价员按照纳入与排除标准独立筛选文献、提取资料和方法学质量评价后,采用Meta-DiSc 1.4和Stata 12.0软件进行Meta分析.结果 最终纳入10个研究,包括1 229例标本.Meta分析结果显示:①SEN合并=0.92[95％CI (0.90,0.94),P=0.019 3]、SPE合并=0.97 [95％CI (0.95,0.98),P＜0.000 1]、+LR合并=23.68 [95％CI (8.71,64.37),P＜0.000 1]、-LR合并=0.10 [95％CI (0.06,0.15),P=0.023 1]和DOR合并=257.16 [95％CI (96.82,683.02),P=0.020 0],SROC曲线下面积(AUC)为0.971 5,Q*指数0.922 3.②分别剔除样本量＜100例、中文研究以及QUADAS评分＞10分的研究行敏感性分析,结果显示剔除文献后各诊断结果稳定,提示结论较为可靠.结论 PCR-SSCP检测rpoB基因突变诊断结核分支杆菌耐利福平具有较高的临床应用价值.“,”Objective To systematically review the diagnostic value of PCR-single-strand conformational polymorphism (PCR-SSCP) method for detecting rpoB gene mutation of rifampin-resistant mycobacterium tuberculosis.Methods Such databases as PubMed,Web of Science,The Cochrane Library (Issue 2,2014),CBM,VIP and WanFang Data were electronically and comprehensively searched for relevant studies on the diagnostic value of PCR-SSCP method for detecting rpoB gene mutation of rifampin-resistant mycobacterium tuberculosis from inception to January 1st,2014.Literature screening according to the inclusion and exclusion criteria,data extraction and methodological quality assessment were completed by two reviewers independently.Meta-analysis was then conducted using Meta-Disc 1.4 and Stata 12.0.Results A total of 10 studies were included involving 1 299 cases.The results of meta-analysis showed SEN=0.92 (95％CI 0.90 to 0.94,P=0.019 3),SPE=0.97 (95％CI 0.95 to 0.98,P＜0.000 1),+LR=23.68 (95％CI 8.71 to 64.37,P＜0.000 1),-LR=0.10 (95％CI 0.06 to 0.15,P=0.023 1),DOR=257.16 (95％CI 96.82 to 683.02,P=0.020 0),and SROC area under the curve (AUC) was 0.971 5,and Q* was 0.922 3.The results of sensitivity analysis (after removing studies with sample size less than 100,Chinese studies and QUADAS more than 10 studies) showed that,the results were stable with reliable conclusion.Conclusion PCR-SSCP method has a fairly high value in the diagnosis of rpoB gene mutation of rifampinresistant mycobacterium tuberculosis.