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目的建立活血止痛软胶囊中的阿魏酸的含量测定方法。方法采用高效液相色谱法,以C18(5μm,4.6mm×250mm)为色谱柱,乙腈-0.085%磷酸溶液(15∶85)为流动相,流速为1.0m L/min,检测波长为316nm。结果方法学考察表明:阿魏酸的进样量在0.0137~0.3415μg范围内峰面积与进样量呈良好的线性关系(r=1),平均回收率为98.3%(n=6),RSD=1.0%;结论本方法较原标准中方法专属性强,更简便、快速、准确,提高和完善了活血止痛软胶囊的质量控制标准。
Objective To establish a method for the determination of ferulic acid in Huoxue Zhitong soft capsule. Methods The mobile phase consisted of C18 (5μm, 4.6mm × 250mm) column, acetonitrile-0.085% phosphoric acid solution (15:85), the flow rate was 1.0m L / min and the detection wavelength was 316nm by high performance liquid chromatography. Results The methodological study showed that the peak area of the sample of ferulic acid in the range of 0.0137-0.3415μg was linear with the injection volume (r = 1), and the average recovery was 98.3% (n = 6). RSD = 1.0%. Conclusion The method is more specific, simpler, faster and more accurate than the original standard method, which improves and improves the quality control standards of Huoxue Zhitong soft capsule.