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AIM To investigate the apoptosis in esophageal cancer cellsinduced by resveratrol,and the relation between thisapoptosis and expression of Bcl-2 and Bax.METHODS:In in vitro experiments,MTT assay was usedto determine the cell growth inhibitory rate.Transmissionelectron microscope and TUNEL staining method were usedto quantitatively and qualitively detect the apoptosis statusof esophageal cancer cell line EC-9706 before and after theresveratrol treatment.Immunohistochemical staining wasused to detect the expression of apoptosis-regulated geneBcl-2 and Bax.RESULTS:Resveratrol inhibited the growth of esophagealcancer cell line EC-9706 in a dose-and time-dependentmanner.Resveratrol induced EC-9706 cells to undergoapoptosis with typically apoptotic characteristics,includingmorphological changes of chromatin condensation,chromatincrescent formation,nucleus fragmentation and apoptoticbody formation.TUNEL assay showed that after thetreatment of EC-9706 cells with resveratrol(10 mmol·L~(-1))for 24 to 96 hours,the AIs were apparently increased withtreated time(P<0.05).Immunohistochemical stainingshowed that after the treatment of EC-9706 cells withresveratrol(10 mmol·L~(-1))for 24 to 96 hours,the PRs of Bcl-2proteins were apparently reduced with treated time(P<0.05)and the PRs of Bax proteins were apparently increased withtreated time(P<0.05).CONCLUSION:Resveratrol is able to induce the apoptosisin esophageal cancer.This apoptosis may be mediated bydown-regulating the apoptosis-regulated gene Bcl-2 and up-regulating the expression of apoptosis-regulated gene bax.
AIM To investigate the apoptosis in esophageal cancer cells induced by resveratrol, and the relation between thisapoptosis and expression of Bcl-2 and Bax. METHODS: In vitro experiments, MTT assay was used to determine the cell growth inhibitory rate. Transmission electron microscope and TUNEL staining method were used to quantitatively and qualitively detect the apoptosis status of esophageal cancer cell line EC-9706 before and after the resveratrol treatment. Immunohistochemical staining was used to detect the expression of apoptosis-regulated gene Bcl-2 and Bax.RESULTS: Resveratrol inhibited the growth of esophageal cancer cell line EC -9706 in a dose-and time-dependentmanner. Resveratrol induced EC-9706 cells to undergoapoptosis with very apoptotic characteristics, including morphological changes of chromatin condensation, chromatincrescent formation, nucleus fragmentation and apoptotic body formation. TUNEL assay showed that after the treatment of EC-9706 cells with resveratrol (10 mmol·L -1) for The AIs were apparently increased with the time of treatment (P <0.05). Immunohistochemical staining showed that after the treatment of EC-9706 cells withresveratrol (10 mmol·L -1) for 24 to 96 hours, the PRs of Bcl-2proteins were apparently reduced with treated time (P <0.05) and the PRs of Bax proteins were apparently increased withtreated time (P <0.05) .CONCLUSION: Resveratrol is able to induce the apoptosis in esophageal cancer. This apoptosis may be mediated bydown- regulating the apoptosis-regulated gene Bcl-2 and up-regulating the expression of apoptosis-regulated gene bax.