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目的探讨脑缺血再灌注后海马区白细胞介素1(IL1β)与促肾上腺皮质激素释放因子(CRF)蛋白表达的诱导关系。方法对Koizumi和Nagasawa法加以改进,采用同侧颈总动脉永久结扎线栓法制备短暂脑缺血大鼠模型,2h后实现大脑中动脉再灌注。假手术组大鼠作为对照组。分别在再灌注后30min及1、2、4、6、12、24h至7d的不同时间取材,应用免疫组化方法标记实验各组大鼠脑海马区组织中IL1β、CRF免疫反应细胞数量。结果IL1β免疫反应细胞在缺血再灌注后海马区从1h(974±393)后开始表达,2h(6107±1884)时达高峰,至7d(66±59)时IL1β免疫反应细胞表达基本消失;CRF蛋白免疫反应细胞从2h(972±184)时开始表达,12h(3744±570)和7d(3963±510)时表达为2次高峰。而且,脑缺血再灌注后海马区IL1β、CRF蛋白表达在时间上具有一定的诱导关系,即IL1β表达早于CRF蛋白。结论此研究提示大鼠脑缺血再灌注后海马区IL1β蛋白的升高表达可诱发内生的CRF蛋白表达增加。
Objective To investigate the induction of interleukin - 1 (IL - 1β) and corticotropin releasing factor (CRF) protein expression in the hippocampus after cerebral ischemia / reperfusion in rats. Methods The methods of Koizumi and Nagasawa were modified. The model of transient cerebral ischemia was established by permanent ligation of the common carotid artery, and the middle cerebral artery was reperfused after 2 hours. Rats in sham operation group served as control group. The cells were harvested at different time points of 30 min, 1, 2, 4, 6, 12, 24 h to 7 d after reperfusion, and the numbers of IL1β and CRF immunoreactive cells in hippocampus tissue of each group were marked by immunohistochemistry. Results The expression of IL1β immunoreactive cells in the hippocampus after 1h (974 ± 393) ischemia reperfusion was peaked at 2h (6107 ± 1884), and disappeared at 7 days (66 ± 59) CRF protein immunoreactive cells began to express at 2h (972 ± 184), reached the second peak at 12h (3744 ± 570) and 7d (3963 ± 510). Moreover, the expression of IL1β and CRF in the hippocampus after cerebral ischemia-reperfusion has a certain induction in time, that is, the expression of IL1β is earlier than the CRF protein. Conclusions This study suggests that elevated expression of IL1β in hippocampus may induce an increase in endogenous CRF protein expression following cerebral ischemia-reperfusion in rats.