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目的 :观察甘草酸二铵的抗肝纤维化作用。方法 :二甲基亚硝胺腹腔注射诱导大鼠肝纤维化模型 ,以甘草酸二铵 7mg/10 0 g体重灌胃治疗 ,每日 1次 ,共 4周 ,并以 γ干扰素为对照药物。观察大鼠体重、肝脾重量等一般情况 ;肝脏苏木精 -伊红染色与丽春红胶原染色 ,分级分期观察肝组织的炎性坏死与胶原纤维沉积变化 ;检测血清肝功能变化与肝组织羟脯氨酸含量。结果 :模型大鼠肝脏胶原纤维间隔形成 ,肝小叶与肝窦内胶原增生沉积明显 ;脾脏明显增大 ;血清 AL T活性升高 ,肝组织羟脯氨酸含量上升。甘草酸二铵可显著降低模型大鼠肝组织羟脯氨酸含量 ,减轻肝脏内胶原纤维增生沉积 ,降低血清 AL T活性。与 γ干扰素比较作用无明显差异。结论 :甘草酸二铵有良好的抗二甲基亚硝胺诱导的大鼠肝纤维化效果 ,其作用机制可能与药物减轻肝脏炎症 ,抑制肝脏羟脯氨酸与胶原生成有关。
Objective: To observe the anti-fibrotic effect of diammonium glycyrrhizinate. METHODS: Rat models of hepatic fibrosis were induced by intraperitoneal injection of dimethylnitrosamine and gavage with diammonium glycyrrhizinate 7 mg/10 0 g body weight once a day for 4 weeks with IFN-γ as a control drug. . Observe the general conditions of body weight, hepatosplenomegaly, etc., liver hematoxylin-eosin staining, and Ponceau red collagen staining; observe inflammatory necrosis and collagen fiber deposition changes in liver tissue in stages; detect serum liver function changes and liver tissue Hydroxyproline content. RESULTS : The collagen fibers of the rat model were formed at intervals, and the proliferation of collagen in the hepatic sinusoids and hepatic sinusoids was evident. The spleen was significantly enlarged; the activity of serum AL T was increased and the content of hydroxyproline in liver tissue was increased. Diammonium glycyrrhizinate can significantly reduce the content of hydroxyproline in the liver tissue of model rats, reduce the deposition of collagen fibers in the liver, and reduce serum AL T activity. There was no significant difference between the effects of IFN-γ and IFN-γ. Conclusion :Diammonium glycyrrhizinate has good anti-dimethylnitrosamine-induced hepatic fibrosis in rats. Its mechanism of action may be related to the drug to reduce hepatic inflammation and inhibit hepatic hydroxyproline and collagen production.