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目的 :探讨渗出性结核性胸膜炎患者外周血T淋巴细胞亚群和活化变化的临床意义。方法 :利用荧光标记—流式细胞仪技术检测T淋巴细胞亚群和CD2 5表达。结果 :10例血清和胸水结核抗体双阳性结核性胸膜炎患者的外周血CT3+ T细胞和CT4 + T细胞的比率明显降低 ,7例单纯胸水结核抗体阳性结核性胸膜炎患者的外周血CD4 + T细胞的比率也明显降低 ,但是 9例血清和胸水结核抗体双阴性结核性胸膜炎患者的外周血CD4 + T细胞的比率只是略有降低。 10例血清和胸水结核抗体双阳性和 7例单纯胸水结核抗体阳性的结核性胸膜炎患者 ,其外周血CD4 + T细胞表面CD2 5的表达无明显变化 ,而 9例血清和胸水结核抗体双阴性结核性胸膜炎患者 ,其外周血CD4 + T细胞表面CD2 5的表达却明显增高。结论 :结核性胸膜炎患者随着病程的进展免疫反应有局限化的趋势
Objective: To investigate the clinical significance of T lymphocyte subsets and activation in peripheral blood of patients with exudative tuberculous pleurisy. Methods: T lymphocyte subsets and CD25 expression were detected by fluorescence-labeled flow cytometry. Results: The ratio of CT3 + T cells and CT4 + T cells in peripheral blood of 10 patients with tuberculous pleurisy and 10 patients with tuberculous pleurisy was significantly lower. The percentage of CD4 + T cells in peripheral blood of 7 patients with pleural fluid tuberculosis positive tuberculous pleurisy However, the ratio of CD4 + T cells in peripheral blood of 9 patients with double negative tuberculous pleurisy was only slightly decreased. There was no significant change in the expression of CD25 on the surface of CD4 + T cells in 10 cases of tuberculous pleurisy with positive serum and pleural fluid tuberculosis antibody and 7 cases of pleural fluid positive tuberculosis antibody. However, 9 cases of serum and pleural fluid tuberculosis double negative tuberculosis In patients with pleurisy, the expression of CD25 on the surface of CD4 + T cells in peripheral blood was significantly increased. Conclusion: There is a limited tendency of immunological reaction in patients with tuberculous pleurisy as the disease progresses