Flow-injection Chemiluminescence Determination of Trace Calf Thymus DNA

来源 :Chinese Chemical Letters | 被引量 : 0次 | 上传用户:xingzhewei1123
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A flow injection procedure for the determination of calf thymus DMA (CT DNA) over the range 2.1x10-6~2.1x10-1 B.g mL-1 is described, based on measurement of the enhanced chemiluminescence emission of rhodamine B-Ce(IV) system, activated by imidazole-HCl buffer solution. This method is highly sensitive, with the linearity range broadened to five orders of magnitude. It has been applied to determine CT DNA in synthetic sample with satisfactory results. A flow injection procedure for the determination of calf thymus DMA (CT DNA) over the range 2.1x10-6 ~ 2.1x10-1 Bg mL-1 is described, based on measurement of the enhanced chemiluminescence emission of rhodamine B-Ce (IV) system, activated by imidazole-HCl buffer solution. This method is highly sensitive, with the linearity range broadened to five orders of magnitude. It has been applied to determine CT DNA in synthetic sample with satisfactory results.
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