论文部分内容阅读
目的 构建抗 HFRS病毒 m Ab 1 A8鼠 /人嵌合 Fab噬菌体抗体表达载体 ,并进行表达 .方法 将抗 HFRS病毒m Ab1 A8的 VH 基因和人重链 CH1基因连接 ,VL 基因和人Cκ 基因连接 ,分别构建了鼠 /人嵌合重链 Fd基因和轻链基因 ,将它们克隆入 p Comb3,构建了鼠 /人嵌合 Fab噬菌体抗体表达载体 .用辅噬菌体 VCSM1 3超感染 ,EL ISA间接夹心法检测抗体活性 .结果 经多种限制性内切酶酶切鉴定载体构建正确 ,ELISA间接夹心法检测超感染上清为阳性 .结论 成功构建了鼠 /人嵌合 Fab噬菌体抗体表达载体 ,并在噬菌体表面表达出可结合 HFRS病毒抗原的鼠 /人嵌合 Fab抗体
OBJECTIVE: To construct and express the anti-HFRSV m Ab 1 A8 murine / human chimeric Fab phage antibody expression vector.Methods The VH gene of anti-HFRS virus m Ab1 A8 was linked to the human heavy chain CH1 gene and the VL gene was linked to the human Cκ gene , The mouse / human chimeric heavy chain Fd gene and light chain gene were constructed respectively and cloned into p Comb3 to construct the mouse / human chimeric Fab phage antibody expression vector. Method was used to detect the activity of the antibody.Results The correct vector was constructed by restriction endonuclease digestion and the supernatant was positive by ELISA sandwich ELISA.Conclusion The mouse / human chimeric Fab phage antibody expression vector was successfully constructed and expressed at The phage surface expresses a murine / human chimeric Fab antibody that binds to HFRS virus antigens