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目的:建立加替沙星的反相高效液相色谱方法,并对其肾损害模型大鼠体内过程进行分析研究。方法:正常和肾损害造模大鼠ig 20 mg·kg-1加替沙星后5,15 min和4 h测定各组织药物浓度,并收集尿液、胆汁与粪便,测定累积排泄率。结果:正常和肾损害造模大鼠ig20 mg·kg-1加替沙星后5,15 min和4 h,快速分布在各组织中,其中肝、肾、小肠、胃分布最多,大脑未测到药物。正常组与肾损害组大鼠ig 20 mg·kg-1加替沙星后48 h,尿液、胆汁与粪便药物累积排泄率分别为(66．2±8．8)％与(71．2±13．6)％,(8．05±3．08)％与(1．62±O.67)％和(3．63±1．65)％与(3．92±1．87)％。结论:加替沙星在肾损害模型大鼠组织分布和尿液的排泄受到影响,通过了解其体内过程,可为临床应用提供参考。
OBJECTIVE: To establish a method for the determination of gatifloxacin by reversed-phase high performance liquid chromatography (HPLC) and to analyze the in vivo process of rat model of renal damage. Methods: The drug concentration in each tissue was determined at 5, 15 min and 4 h after ig 20 mg · kg -1 gatifloxacin injection in normal and renal injured rats. Urine, bile and feces were collected and the cumulative excretion rate was determined. RESULTS: Normal and renal damage rats were rapidly established in 5, 15 min and 4 h after ig 20 mg · kg -1 gatifloxacin injection, with the distribution of liver, kidney, small intestine and stomach being the highest, while the brain was not measured To the drug. The cumulative excretion rates of urinary, bile and stool samples were (66.2 ± 8.8)% and (71.2%, respectively) at 48 h after ig 20 mg · kg-1 gatifloxacin injection (1.62 ± 0.67)% and (3.63 ± 1.65)% and (3.92 ± 1.87)%, respectively . Conclusion: Gatifloxacin can affect the tissue distribution and excretion of urine in rats with renal impairment, and provide a reference for clinical application by understanding its in vivo process.