【摘 要】
：
The precise regulation of microRNA (miRNA) biogenesis is crucial for plant development,which requires core microprocessors and many fine tuners to coordinate th
【机 构】
：
Department of Systems Biology, College of Life Science and Biotechnology, Yonsei University, Seou112
论文部分内容阅读
The precise regulation of microRNA (miRNA) biogenesis is crucial for plant development,which requires core microprocessors and many fine tuners to coordinate their miRNA processing activity/ specificity in fluctuating cellular environments.During de-etiolation,light triggers a dramatic accumulation of core microprocessors and primary miRNAs (pri-miRNAs) but decreases pri-miRNA processing activity,resulting in relatively constant miRNA levels.The mechanisms underlying these seemingly contradictory regulatory changes remain unclear.In this study,we identified forkhead-associated domain 2 (FHA2) as a light-stabilized suppressor of miRNA biogenesis.We found that FHA2 deficiency increased the level of mature miRNAs,accompanied by a reduction in pri-miRNAs and target mRNAs.Biochemical assays showed that FHA2 associates with the core microprocessors DCL1,HYL1,and SE,forming a complex to suppress their pri-miRNA processing activity.Further analyses revealed that FHA2 promotes HYL1 binding but inhibits the binding of DCL1-PAZ-RNase-RNA-binding domains (DCL1-PRR) to miRNAs,whereas FHA2 does not directly bind to these RNAs.Interestingly,we found that FHA2 protein is unstable in the dark but stabilized by light during de-etiolation.Consistently,disruption of FHA led to defects in light-triggered changes in miRNA expression and reduced the survival rate of deetiolated seedlings after prolonged light deprivation.Collectively,these data suggest that FHA2 is a novel light-stabilized suppressor of miRNA biogenesis and plays a role in fine-tuning miRNA processing during de-etiolation.
其他文献
Higher plants evolved multiple innate immune systems to combat pathogens present in their living environment.However, activation of the complex immune responses
For decades, practical limitations and cost have created a bias in plant genetic studies toward species that tolerate self pollination.An important work by Chen
The complexity of the epigenome landscape and transcriptional regulation is significantly increased during plant polyploidization,which drives genome evolution
Dear Editor,rnUrine passes through the entire kidney and urinary tract system starting from the glomerulus and ending to the urethra.Cells in the kidney and uri
High fidelity two-qubit gates are fundamental for scaling up the superconducting qubit number.We use two qubits coupled via a frequency-tunable coupler which ca
Coherent photon source is an important element that has been widely used in spectroscopy,imaging,detection,and teleportation in quantum optics.However,it is sti
Metabolic regulation has been proven to play a critical role in T cell antitumor immunity.However,cholesterol metabolism as a key component of this regulation r
We find by the wavelet transform that the classical plane light wave of linear polarization can be decomposed into a series of discrete Morlet wavelets.In the t
阐述了物理学科核心素养的概念内涵，论述物理实验教学中培养学生核心素养的作用意义，基于物理实验教学现状问题分析，探讨实验教学中培养学生核心素养的有效措施。
Abscisic acid (ABA) transport plays an important role in systemic plant responses to environmental factors.However,it remains largely unclear about the precise