论文部分内容阅读
以硫酸奎宁为内标，反相ＨＰＬＣ－ＵＶ法定量测定血浆中普萘洛尔的含量。固定相为Ｓｐｈｅｒｉｓｏｒｂ５ＯＤＳ（２），流动相为甲醇－水－０．２ｍｏｌ／Ｌ磷酸二氢钾（１００：１５：１）。紫外检测波长为２３２ｎｍ。检测浓度可低达１０ｎｇ／ｍｌ。线性范围为１０～２００ｎｇ／ｍｌ（ｒ＝０．９９９７）。本法灵敏，准确，简便，可用于血浆普萘洛尔测定及其药代动力学研究。
Quinine sulfate as an internal standard, reverse phase HPLC-UV method for quantitative determination of propranolol in plasma. The stationary phase was Spherisorb 5 ODS (2) and the mobile phase was methanol-water-0.2 mol / L potassium dihydrogen phosphate (100: 15: 1). UV detection wavelength of 232nm. Test concentrations can be as low as 10ng / ml. The linear range was 10 ~ 200ng / ml (r = 0.9997). Sensitive, accurate and simple, this method can be used for the determination of plasma propranolol and its pharmacokinetics.