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目的观察分析乙酸铅、硫酸铝和甲醛3种化学物对血脑屏障(blood-brain barrier,BBB)通透性的作用影响。方法利用血脑屏障模型(blood-brain barrier model,BBBM)进行3种化学物通透性试验,计算通透系数(Permeability Coefficient,Pe)用以反映血脑屏障通透性变化情况;伊文氏蓝比色法测定3种化学物对Wistar试验大鼠血脑屏障通透性作用影响。对体内、外测试结果一致性进行比较分析。结果求得乙酸铅、硫酸铝和甲醛3种化学物Pe值分别为(2.38±0.54)×10-3、(2.07±0.33)×10-3和(1.11±0.28)×10-3cm/min,其中乙酸铅和硫酸铝组Pe值与对照组[(1.06±0.17)×10-3cm/min]比较,差异具有统计学意义(P<0.01);动物试验结果显示,乙酸铅、硫酸铝和甲醛对试验大鼠大脑皮质血脑屏障通透性影响增高和对照组比较差异均有统计学意义(P<0.05)。结论乙酸铅和硫酸铝两种化学物体内外均能对血脑屏障通透性产生显著影响。甲醛在体内可对血脑屏障通透性产生显著影响。甲醛在体外对血脑屏障通透性产生影响有待进一步证实。
Objective To investigate the effects of lead, aluminum sulfate and formaldehyde on the permeability of blood-brain barrier (BBB). Methods Permeability Coefficient (Pe) was used to reflect the change of blood-brain barrier permeability by using blood-brain barrier model (BBBM) Colorimetric determination of three chemicals on Wistar rats blood - brain barrier permeability. To compare the consistency of test results in vivo and in vitro. Results The Pe values of lead, aluminum sulfate and formaldehyde were (2.38 ± 0.54) × 10-3, (2.07 ± 0.33) × 10-3 and (1.11 ± 0.28) × 10-3cm / min, The Pe value of lead acetate and aluminum sulfate group was significantly lower than that of the control group [(1.06 ± 0.17) × 10-3 cm / min] (P <0.01). The results of animal experiments showed that lead acetate, aluminum sulfate and formaldehyde There was a significant difference between the control group and the control group (P <0.05). Conclusion Both lead acetate and aluminum sulfate can affect blood-brain barrier permeability both in vitro and in vivo. Formaldehyde can have a significant effect on the permeability of the blood-brain barrier in the body. Formaldehyde in vitro affect the permeability of the blood-brain barrier needs further confirmation.