论文部分内容阅读
目的:研究Ku蛋白表达与膀胱癌发生、进展的关系。方法:应用SP法对98例行经尿道膀胱肿瘤电切术(TURBT)、根治性膀胱切除术的膀胱组织标本进行免疫组织化学染色;应用形态计量学分析方法结合临床相关病理资料分析Ku蛋白表达与膀胱癌肿瘤相关指标的关系。结果:98例膀胱癌组织中Ku蛋白总阳性表达数为79例(80.61%)。67例非肌层浸润性膀胱癌组织中Ku蛋白阳性表达数为60例(89.55%),31例肌层浸润性膀胱癌中Ku蛋白阳性表达数为19例(61.29%),两者差异有显著统计学意义(P<0.01)。图像分析结果:肌层浸润性膀胱癌组(2 453.05±221.24)和非肌层浸润性膀胱癌组(4 349.41±307.20)之间积分光密度的差异有显著统计学意义(P<0.01)。肿瘤组织(3 605.67±1 024.80)与癌旁组织(396.84±60.24)之间积分光密度的差异度亦有显著统计学意义(P<0.01)。结论:Ku蛋白可能在膀胱癌进展的过程中发挥着重要作用。早期Ku蛋白表达增加是膀胱癌发生的一个重要因素。晚期Ku蛋白表达下调与膀胱癌的侵袭性密切相关。
Objective: To study the relationship between Ku protein expression and the occurrence and progression of bladder cancer. Methods: 98 cases of TURBT and radical cystectomy were examined by immunohistochemical staining with SP method. The expression of Ku protein was analyzed by morphometric method and clinically relevant pathological data And bladder cancer related indicators. Results: The total positive expression of Ku protein in 98 cases of bladder cancer was 79 cases (80.61%). The positive expression of Ku protein in 67 cases of non-invasive invasive bladder cancer was 60 cases (89.55%). The positive expression of Ku protein in 31 cases of myometrial invasive bladder cancer was 19 cases (61.29%), the difference was Significant statistical significance (P <0.01). The results of image analysis showed that the difference of integral optical density between myometrial invasive bladder cancer group (2 453.05 ± 221.24) and non-muscle invasive bladder cancer group (4 349.41 ± 307.20) was statistically significant (P <0.01). The difference of integral optical density between tumor tissues (3 605.67 ± 1024.80) and adjacent tissues (396.84 ± 60.24) was also statistically significant (P <0.01). Conclusion: Ku protein may play an important role in the progression of bladder cancer. Early Ku protein expression increased bladder cancer is an important factor. The expression of late Ku protein is closely related to the invasion of bladder cancer.