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目的:探讨槐耳清膏对人肺腺癌A549细胞和A549/DDP细胞增殖的抑制影响及其顺铂耐药细胞A549/DDP的逆转作用。方法:以体外培养的人肺腺癌A549及A549/DDP细胞为实验模型,采用MTT法测定不同浓度槐耳清膏对A549细胞及A549/DDP细胞的增殖抑制率,并运用流式细胞术检测顺铂联合槐耳清膏对A549/DDP细胞的变化,以及增敏作用效应。结果:槐耳清膏对A549细胞及A549/DDP细胞的增殖有明显抑制作用,随着药物浓度的增加,其抑制率逐渐增加,具有明显的量-效关系(P<0.05)。顺铂40μmol/L作用于A549及A549/DDP细胞48 h后,其凋亡率分别为(27.61±3.45)%和(17.93±1.73)%,两组间有统计学差异(P<0.01)。槐耳清膏1 mg/m L作用于人肺腺癌A549和A549/DDP细胞40 h后,再以相同浓度顺铂诱导,A549细胞凋亡率为(32.35±2.68)%,A549/DDP细胞凋亡率为(30.01±3.65)%,两组间无统计学意义,但与单独DDP作用相比,差异具有显著意义(P<0.01)。结论:槐耳清膏能显著抑制人肺腺癌A549及A549/DDP细胞的增殖,诱导细胞凋亡,还可以增加A549/DDP细胞对顺铂的敏感性。
OBJECTIVE: To investigate the effects of Huaierqing cream on the proliferation of human lung adenocarcinoma A549 cells and A549 / DDP cells and the reversal of cisplatin-resistant A549 / DDP cells. Methods: Human lung adenocarcinoma A549 and A549 / DDP cells cultured in vitro were used as experimental models. MTT assay was used to determine the inhibitory rates of proliferation of A549 cells and A549 / DDP cells with different concentrations of Chinese Yau ear cream. Flow cytometry The effect of cisplatin combined with Huaierqing plaster on A549 / DDP cells and sensitization effect. Results: The Hua-hui cream could significantly inhibit the proliferation of A549 cells and A549 / DDP cells. With the increase of the drug concentration, the inhibition rate of the Hua-hui-qi paste increased gradually with a significant dose-response relationship (P <0.05). The apoptosis rates of A549 and A549 / DDP cells treated with cisplatin (40μmol / L) for 48 h were (27.61 ± 3.45)% and (17.93 ± 1.73)%, respectively. There was a significant difference between the two groups (P <0.01). A549 / DDP cells were treated with IPTG 1 mg / m L for 40 h and then induced with the same concentration of cisplatin, the apoptotic rates of A549 cells were (32.35 ± 2.68)%, The apoptotic rate was (30.01 ± 3.65)%. There was no significant difference between the two groups, but the difference was significant compared with the effect of DDP alone (P <0.01). Conclusion: The Chinese Yau ear cream can significantly inhibit the proliferation of human lung adenocarcinoma A549 and A549 / DDP cells and induce cell apoptosis, and can also increase the sensitivity of A549 / DDP cells to cisplatin.