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改“425”制剂（中药当归提取物）为口服型，并伍用鲨鱼软骨粉（SCP），制成“425”合剂，用以研究感染血吸虫3周的BALB／c小鼠服用“425”合剂后4周诱导的抗肝内虫卵肉芽肿（SEG）效应及机制。测定小鼠肝内SEG大小，用EtnA检测小鼠血清抗体IgG及其亚类IgGI水平（OD均值）；采用流式细胞仪测定小鼠脾脏T细胞亚群的细胞均数和比值。服用“425”合剂（组Ⅰ）和单用SCP（组Ⅲ）小鼠的SEG大小显著小于感染对照鼠（组Ⅳ），组Ⅰ、组Ⅱ（单用“425”制剂）和组Ⅲ的SEG减小车之间无显著性差异；组Ⅰ和组Ⅱ的IgG和ISG1水平均显著高于组Ⅲ和组Ⅳ者；组Ⅰ和组Ⅲ的CD4+／CD8+比值（1．70－1．88）明显高于组Ⅱ（0．95）。实验结果表明，“425”合剂能诱导明显的抗肉芽肿效应，其机制与体液免疫和细胞免疫密切相关；SCP能非常显著诱导细胞免疫效应，特别是具有抗肝内纤维化作用；“425”制剂能明显诱导体液免疫效应。‘本项研究尚未见有类似报道。
The modified “425” preparation (Chinese Angelica extract) was taken orally and shaved cartilage powder (SCP) was used to make a “425” mixture for the study of “425” mixture in BALB/c mice infected with schistosome for 3 weeks. Induction of anti-hepatic egg granuloma (SEG) effect and mechanism after 4 weeks. The mice’s hepatic SEG size was measured. The serum antibody IgG and its subclass IgGI levels (OD mean) were detected with EtnA. The mean and ratio of the T cell subsets of the mouse spleen were determined by flow cytometry. The SEG size of mice taking “425” mixture (group I) and SCP alone (group III) was significantly smaller than that of infected control mice (group IV), group I, group II (single “425” formulation) and group III SEG There was no significant difference between the reduced cars; the levels of IgG and ISG1 in group I and group II were significantly higher than those in groups III and IV; the ratio of CD4+/CD8+ in group I and group III (1.70-1.88) Significantly higher than group II (0.95). The experimental results show that the “425” mixture can induce significant anti-granulomatous effects, and its mechanism is closely related to humoral immunity and cellular immunity; SCP can very significantly induce cellular immune effects, in particular, has anti-intrahepatic fibrosis; “425” Formulations can significantly induce humoral immune effects. ‘This study has not seen similar reports.