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生物学效应重组的人γ—干扰素(rIFN—γ)是由克隆的大肠杆菌纯化获得,含139个氨基酸的单体,分子量约17000,rIFN—γ—1b为美国Genentech公司的产品,其比活性为3×10~7u/mg蛋白。 IFNs能够诱导宿主细胞产生抗病毒状态,从而干扰病毒感染和复制的不同阶段。IFN还可通过诱导和活化2’、5’—寡腺苷酸合成酶通路和真核生物蛋白合成起动因子蛋白激酶磷酸酶通路抑制病毒的拷贝。在淋巴因子活化Mφ导致抗菌活力增强的过程中
The biological effect recombinant human γ-interferon (rIFN-γ) is obtained by cloning E. coli purified, containing 139 amino acid monomer, molecular weight of about 17000, rIFN-γ-1b Genentech company of the United States, the ratio Activity of 3 × 10 ~ 7u / mg protein. IFNs can induce host cells to develop an antiviral status that interferes with the different stages of viral infection and replication. IFN can also inhibit viral copies by inducing and activating the 2 ’, 5’-oligoadenylate synthase pathway and the eukaryotic protein synthesis activator protein kinase phosphatase pathway. Activation of Mφ by lymphokines leads to increased antimicrobial activity