论文部分内容阅读
The gene of RNA-dependent RNA polymerase(RdRP) was cloned by RT-PCR from Sugarcane yellow leaf virus-Fuzhou isolate(CHN-FJ1) and then cloned into pMD18-T vector.The sequence showed that the fragment comprised 1 212 nucleotides including part gene of ORF1 and ORF2.The ORF2 was involved the RdRP gene consisted of 995 nucleotides and encoded putative protein of 331 amino acids.Compared the nucleo-tide sequence and encoded putative protein of CHN-FJ1 with the other isolates from different countries,they shared the homology above 92.0%.Phylogenetic tree suggested that the sixteen isolates were classified into four types according to the amino acid sequence of the RdRP.One of the groups contained CHN-FJ1 and the other isolates from China,American,Brazil,Australia and Colombia;however,there was the closest relation between CHN-FJ1 and BRA-YL1 isolate from Brazil.
The gene of RNA-dependent RNA polymerase (RdRP) was cloned by RT-PCR from Sugarcane yellow leaf virus-Fuzhou isolate (CHN-FJ1) and then cloned into pMD18-T vector. The sequence showed that the fragment was 1 212 nucleotides including part gene of ORF1 and ORF2. The ORF2 was involved in the formation of the RdRP gene composed of 995 nucleotides and encoded putative protein of 331 amino acids. Compared the nucleo-tide sequence and encoded putative protein of CHN-FJ1 with the other isolates from different countries, they shared the homology above 92.0%. Phylogenetic tree suggested that the sixteen isolates were classified into four types according to the amino acid sequence of the RdRP. One of the groups contained CHN-FJ1 and the other isolates from China, American, Brazil, Australia and However, there was the closest relation between CHN-FJ1 and BRA-YL1 isolate from Brazil.