论文部分内容阅读
目的了解吉林省流感病毒的流行病学特征,为流感防控措施的制定提供科学依据。方法采集哨点医院流感样病例的鼻咽拭子标本,用实时荧光定量PCR方法进行病毒核酸检测,流感病毒核酸阳性标本接种马丁达比犬肾细胞(madin-daiby canine kidney cells,MDCK)细胞进行病毒分离,对分离后的毒株用红细胞凝集试验(hemagglutination test,HA)及红细胞凝集抑制试验(hemagglutination inhibitoin test,HI)进行型别鉴定。结果 2010-2014年监测年度报告的流感样病例比例差异有统计学意义(X~2=8 969.11,P<0.001)。2010-2014年监测年度共采集流感样病例患者鼻咽拭子标本22 703份,分离得到流感病毒毒株共1 103株,总毒株分离率为4.86%。2011-2012年以B型流感病毒为流行优势株,其他三个监测年度都以A型流感病毒为流行优势株,流感流行优势株的具体型别与全国其他省份的流感流行优势株基本一致。结论 2010-2014年流感样病例就诊比例有逐年升高的趋势,主要感染人群是儿童和少年,应加强相应的防控措施并积极推广流感疫苗的接种。
Objective To understand the epidemiological characteristics of influenza virus in Jilin Province and provide a scientific basis for the development of influenza prevention and control measures. Methods Nasopharyngeal swab specimens were collected from sentinel hospital for influenza-like illness. Real-time PCR was used to detect the virus nucleic acid. Influenza virus positive samples were inoculated with madin-daiby canine kidney cells (MDCK) The virus was isolated and the isolated strains were identified by hemagglutination test (HA) and hemagglutination inhibitoin test (HI). Results There were significant differences in the proportion of flu-like cases in the annual reports for 2010-2014 (X 2 = 8 969.11, P <0.001). A total of 22 703 samples of nasopharyngeal swabs were collected during the 2010-2014 surveillance year. A total of 1 103 strains of influenza viruses were isolated and the total isolates were isolated with a rate of 4.86%. Influenza B virus was the predominant strain in 2011-2012, and Influenza A virus was the predominant strain in other three monitoring years. The specific types of influenza predominant strains were basically the same as those prevailing in other provinces in the country. Conclusion The proportion of flu-like cases from 2010 to 2014 has been increasing year by year. The main infectious groups are children and adolescents. Corresponding prevention and control measures should be strengthened and flu vaccination should be actively promoted.