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目的观察灯盏花素对体外培养的人类创伤性瘢痕成纤维细胞生长、凋亡的影响,寻找新的治疗瘢痕的有效药物。方法以四甲基偶氮唑(MTT)法和流式细胞仪Annexin V/PI双染色法检测各浓度组灯盏花素对成纤维细胞增殖及凋亡的影响。结果各浓度组灯盏花素对体外培养的瘢痕成纤维细胞生长增殖具有明显的抑制作用(F=3.309,P<0.01)。与对照组相比,80、100、150μmol/ml浓度组对瘢痕成纤维细胞增殖抑制具有显著差异性(P=0.015、0.006、0.000<0.05),并且呈剂量依赖性。IC50处,瘢痕成纤维细胞凋亡率与未加药组瘢痕成纤维细胞相比,差异具有显著性(P<0.05)。结论灯盏花素对瘢痕成纤维细胞的增殖具有显著的抑制作用,并且能够诱导其发生凋亡,为寻找有效治疗增生性瘢痕的药物提供了新的思路。
Objective To observe the effect of breviscapine on the growth and apoptosis of human traumatic scar fibroblasts cultured in vitro and to find a new effective drug for the treatment of scar. Methods The effects of Breviscapine on the proliferation and apoptosis of fibroblasts were detected by MTT assay and flow cytometry Annexin V / PI double staining. Results Breviscapine significantly inhibited the proliferation and proliferation of scar fibroblasts in vitro (F = 3.309, P <0.01). Compared with the control group, the inhibition of the proliferation of scar fibroblasts in 80,100,150μmol / ml group was significantly different (P = 0.015,0.006,0.000 <0.05), and in a dose-dependent manner. IC50, scar fibroblasts apoptosis rate compared with the untreated group scar fibroblasts, the difference was significant (P <0.05). Conclusion Breviscapine has a significant inhibitory effect on the proliferation of scar fibroblasts and can induce the apoptosis of scar fibroblasts, which provides a new idea for finding effective drugs for the treatment of hypertrophic scars.