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目的 探讨干细胞因子 (StemCellFactor ,SCF)促进骨髓间充质干细胞 (MSCs)增殖及促进其向心肌细胞分化的作用。方法 SCF对MSCs预处理 ,流式细胞术测定第 4代MSCs的细胞周期 ,DAPI -MSCs在体外模拟的心肌微环境下培养。用数码显微摄像及免疫荧光技术分别记录和检测心肌特异性肌节肌球蛋白重链 (MHCα/β)、肌钙蛋白T(TroponinT ,TnT)的表达 ,分析MSCs向心肌细胞分化的百分率。结果 实验组的G0 /G1期细胞百分率均显著低于对照组 (P <0 0 1) ,S期、G2 /M期细胞百分率及PI值均显著高于对照组 (P <0 0 1)。在共培养的第 2、3、4、5d ,SCF组表达MHC的DAPI -MSCs阳性百分率分别为 (1 96± 0 3 3 ) %、(4 76±0 3 2 ) %、(5 15± 0 42 ) %、(8 5 2± 0 45 ) % ,显著高于对照组的 (0 5 2± 0 2 1) %、(1 2 5± 0 47) %、(1 75± 0 2 8) %、(3 64± 0 3 2 ) % (P<0 0 1) ;在共培养的第 3、4、5d表达TnT的DAPI -MSCs阳性百分率分别为 (1 10± 0 15 ) %、(2 64± 0 18) %、(5 43± 0 2 4) %均显著高于对照组的 0、(1 2 7± 0 13 ) %、(1 3 2± 0 2 5 ) % (P <0 0 1)。结论 SCF对MSCs具有促增殖、促分化的作用。
Objective To investigate the effects of stem cell factor (SCF) on the proliferation of bone marrow mesenchymal stem cells (MSCs) and their differentiation into cardiomyocytes. Methods MSCs were pretreated with SCF. The cell cycle of MSCs was detected by flow cytometry. DAPI-MSCs were cultured in myocardial microenvironment simulated in vitro. The myocardial specific myosin heavy chain (MHCα / β) and Troponin T (TnT) expression were recorded and detected by digital microscope and immunofluorescence. The percentage of MSCs differentiated into cardiomyocytes was analyzed. Results The percentages of cells in G0 / G1 phase in experimental group were significantly lower than those in control group (P <0.01). The percentage of cells in S phase and G2 / M phase were significantly higher than those in control group (P <0.01). On the 2nd, 3rd, 4th and 5th day of co-culture, the positive percentage of DAPI-MSCs expressing MHC in SCF group were (96 ± 033)%, (476 ± 032)% and 42.5% and 85.2%, respectively, which were significantly higher than that of the control group (0 52 ± 0 2 1)%, (1 2 5 ± 0 47)% and 1 75 ± 0 2 8% (P <0.01). The positive percentage of DAPI-MSCs expressing TnT on the 3rd, 3rd and 5th day of co-culture were (1 10 ± 0 15)%, (2 64 (0 43 ± 0 2 4)% were significantly higher than those in the control group (0 27 ± 0 13%, (1 3 2 ± 0 2 5)%, P 0 01 ). Conclusion SCF can promote the proliferation and differentiation of MSCs.