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目的观察小檗碱(berberine,Ber)对清醒家兔脑电图(EEG)及功率谱的影响 ,探讨Ber改善学习记忆的神经生物学机制。方法利用慢性埋藏电极记录清醒家兔海马和脑皮层EEG及功率谱。结果Ber1～4mg·kg-1iv能使清醒家兔脑皮层区EEG出现持续低振幅快波 ,其主频率功率明显减少 ,并使海马区EEG出现持续且有规律的θ节律 ,海马区主频率从δ段右移至θ段 ,主频率功率明显减少。Ber2mg·kg-1iv可逆转氢溴酸东莨菪碱0.3mg·kg-1iv引起的高振幅慢波和主频率增高的作用 ,同样家兔侧脑室每只注射Ber0.05mg 所引起的EEG及功率谱变化亦可被氢溴酸东莨菪碱0.2mg·kg-1iv所逆转 ,而不能通过血脑屏障的丁溴酸东莨菪碱0.4mg·kg-1iv却无此作用。结论Ber通过影响中枢胆碱能系统在海马产生θ节律 ,减低皮层功率 ,可能是其改善和促进学习记忆的神经生物学机制之一。
Objective To observe the effects of berberine (Ber) on the electroencephalogram (EEG) and power spectrum of awake rabbits and to explore the neurobiological mechanism of Ber in improving learning and memory. Methods Chronic burial electrodes were used to record the EEG and power spectrum of hippocampus and cortex in conscious rabbits. Results Ber1 ~ 4mg · kg-1iv could make the EEG in the cortex of conscious rabbits have a continuous low-amplitude fast wave with a significant decrease in the main frequency power and a continuous and regular θ rhythm in the EEG of the hippocampus. The main frequency in the hippocampus δ segment right to θ, the main frequency power decreased significantly. Ber2mg · kg-1iv reverses scopolamine hydrobromide 0.3mg · kg-1iv caused by high amplitude slow wave and the main frequency of increase, the same rabbit lateral ventricle injection of Ber0.05mg each caused by EEG and power spectrum changes also It could be reversed by scopolamine hydrobromide 0.2mg · kg-1iv, but could not pass the BBB scopolamine 0.4mg · kg-1iv but no such effect. Conclusions Ber is one of the neurobiological mechanisms that ameliorate and promote learning and memory by affecting the central rhythm of the central cholinergic system in the hippocampus and reducing the cortical power.