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目的探讨雌激素在大鼠子宫中抗缺血再灌注损伤的作用。方法清洁级Wistar大鼠50只分为对照组、低雌激素组、高雌激素组、还原型谷胱甘肽(GSH)组和联合组各10只,采用线栓法建立大鼠子宫缺血再灌注损伤模型,缺血30min再灌注1 h后取大鼠子宫组织,利用分光光度计测量大鼠子宫组织匀浆中超氧化物歧化酶(SOD)值、丙二醛(MDA)值,免疫组化染色法(SABC)检测Bcl-2、Bax、caspase-3蛋白的表达。结果雌激素显著降低了缺血再灌注损伤组织中MDA含量,提高了组织中SOD活力。对照组Bax蛋白表达最高,Bcl-2蛋白表达最低;经雌激素、GSH作用后的各组中Bax蛋白表达下降,Bcl-2蛋白表达升高,以联合组Bax蛋白表达最低、Bcl-2蛋白表达最高。Caspase-3于对照组表达最高,经雌激素、GSH作用后的各组表达降低,与对照组相比差异有统计学意义(P<0.01)。结论雌激素对大鼠子宫缺血再灌注损伤具有保护作用。
Objective To investigate the role of estrogen in anti-ischemia-reperfusion injury in rat uterus. Methods Fifty clean Wistar rats were divided into control group, low estrogen group, high estrogen group, reduced glutathione (GSH) group and 10 combined group. The model of reperfusion injury was established. The uterus was harvested 1 h after ischemia and 30 min reperfusion. The contents of superoxide dismutase (SOD) and malondialdehyde (MDA) in uterine tissue were measured by spectrophotometer. The expression of Bcl-2, Bax and caspase-3 proteins were detected by SABC staining. Results Estrogen significantly reduced the content of MDA in ischemia-reperfusion injury tissues and increased the activity of SOD in the tissues. The expression of Bax protein and Bcl-2 protein were the highest in the control group and the lowest in Bcl-2 protein group; the expression of Bax protein and Bcl-2 protein were decreased in the groups treated with estrogen and GSH, The highest expression. The expression of Caspase-3 in the control group was the highest, and the expression of Caspase-3 in the control group was decreased after estrogen and GSH treatment. The difference was statistically significant compared with the control group (P <0.01). Conclusion Estrogen has a protective effect on wistar rats with ischemia-reperfusion injury.