论文部分内容阅读
目的 建立快速分离成人成肌细胞的新方法。方法 改进成肌细胞分离方法,即缩短分离时间并增加分离细胞数。结果 (1)新分离法组织离体到细胞接种所需的时间为2.1±0.52 h,与传统分离法6.8±0.67 h比较,细胞分离所需时间显著缩短(P<0.01)。(2)新分离法每克组织分离获取的细胞数为(8.2±0.88)×10~6较传统分离法(3.0±1.071×10~6显著增加(P<0.01),且纯化后的细胞Desmin(+)率>97％。(3)新分离法和传统分离法分离细胞在生长液中生长的倍增时间分别为25.57h和32.39h,即新方法使成肌细胞倍增时间提前了约7h。(4)新方法和叫方法获取细胞的成肌分化率分别为90.8±1.14％和90.4±0.85％,无显著差异(P>0.05)。结论 新分离法能快速分离获取纯度高倍增快的成人成肌细胞。
Objective To establish a new method for rapid isolation of adult myoblasts. Methods Methods to improve myoblasts separation, ie to shorten the separation time and increase the number of isolated cells. Results (1) The time required for cell isolation in vitro was 2.1 ± 0.52 h in vitro. The time required for cell isolation was significantly shorter than that in the conventional isolation method (6.8 ± 0.67 h) (P <0.01). (2) The number of cells isolated per gram of tissue by the new isolation method was (8.2 ± 0.88) × 10 ~ 6 (3.0 ± 1.071 × 10 ~ 6) significantly higher than that of the traditional separation method (P <0.01) (+)> 97%. (3) The doubling time of newly isolated cells and traditional separation cells growing in the growth solution were 25.57h and 32.39h respectively, that is, the new method promoted the doubling time of myoblasts about 7h. (4) The myogenic differentiation rates of the cells obtained by the new method and the method were 90.8 ± 1.14% and 90.4 ± 0.85%, respectively, no significant difference (P> 0.05) .Conclusion The new separation method can rapidly separate and obtain the adult with high purity Myoblasts.