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目的研究DNA光修复酶(EC 4.1.99.3)对紫外光幅射产生的嘧啶二聚体修复的作用机制.方法利用UV-V is光谱等方法检测不同还原程度的FDA与酶活力的关系.结果辅基FAD在还原状态酶活性最高,自由基状态次之,氧化状态活性最低.氧化状态下的酶,30℃以上会发生部分变性,但不影响其原有的活性.辅基MTHF的存在起到提升酶活性的作用.结论此研究结果可对DNA光修复酶在今后的临床应用中提供参考.“,”Aim To study the mechanism of Escherichia coli DNA photolyase(EC 4.1.99.3) in repairing the ultraviolet induced pyrimidine dimmer lesions in DNA.Methods UV-Vis Specturum was used to measure the relationships between the enzyme activity and different state flavin ademine denucleotide(FAD).Results It was shown that the enzyme activity was the highest when FAD chromophore was in reduced state and lowest when it was in oxidated state,while the activity ranked between when the enzyme was in radical state.Partial denature was observed when the enzyme was in oxidated state and under over 30℃,yet the enzyme activity remained unchanged.The presence of MTHF chromophore enhanced the enzyme activity.Conclusion These findings provide clues for clinical application of DNA photolyase in the future.