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粪类圆线虫具有不同的发育途径:幼虫排出宿主体外后可以直接发育成感染性幼虫(L3i),也可以间接发育为自生生活型成虫,而其后代又可发育至L3i。本文就粪类圆线虫幼虫外周神经元ASF和ASI对发育途径的调控作用进行了观察。 第一期幼虫L1分离自犬粪便,或从其它供体空肠中获得已孵化的幼虫。l1封固于含1-苯氧基-2-丙醇(作为麻醉剂)的琼脂板上。实验组用激光微束照射虫体的神经元
Stool worms have different developmental pathways: After larvae excreted in vitro can develop directly into infectious larvae (L3i), can also indirectly develop into life-form adults, and their offspring can develop to L3i. In this paper, the regulatory effect of ASF and ASI on the developmental pathways of C. elegans larvae was observed. The first larvae L1 are isolated from canine stool or hatched larvae are obtained from other donor jejunum. l1 was mounted on agar plates containing 1-phenoxy-2-propanol as anesthetic. The experimental group irradiated the neurons of the parasite with laser microbeam