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用连续强光或低光驯化后再转到强光下抑制的方法,比较了杂种F_1(3037/02428、Xiandang/02428)及其亲本的光抑制差异,并用对光抑制敏感品种Mars作为对照,结果看出: 1)在连续强光(1680μE·m~(-2)s~(-1))下照射5.5小时,杂种3037/02428 F_1及其亲本02428光合速率分别抑制了17.8％和36.0％,而对光抑制敏感的对照品种Mars却抑制了61.89％(表1)。 2)经低光(1/3自然光)驯化后的叶片,再用强光(2620μE·m~(-2)s~(-1))照射90分钟使其抑制,再在低光(250μE·m~(-2)s~(-1))下恢复210分钟,两个杂种和亲本02428光合速率变化与未经驯化的(在自然光下生长的)对照没有明显的差异(图2)。而两个亲本3037、Xiandans和品种Mars经低光驯化后光合速率变化与对照比较,差异显著。经低光驯化者,光合速率恢复程度不如未驯化者,且在给以30分钟黑暗后,放出的CO_2也多。这表明,两个杂种F_1和02428对光抑制敏感性小,对光的适应能力比3037、Xiandang和Mars为强,杂种F_1的这一特性是来自亲本02428。
The photosynthesis difference of hybrid F_1 (3037/02428, Xiandang / 02428) and its parents was compared with that of domesticated plants by using light or low light acclimation and then transferred to light suppression. The results showed that photosynthetic rate of hybrid 3037/02428 F_1 and its parent 02428 were inhibited by 17.8% and 36.0%, respectively, after being irradiated for 5.5 hours under continuous bright light (1680μE · m -2 s -1) , Whereas maize Mars, which is sensitive to photosystem inhibition, suppressed 61.89% (Table 1). 2) The leaves were domesticated with low light (1/3 natural light) and then irradiated with strong light (2620μE · m -2 s -1) for 90 minutes to inhibit them. In low light (250μE · (-2) s ~ (-1)) for 210 minutes. There was no significant difference in photosynthetic rate between the two hybrids and the parent 02428 compared with the untargeted (grown in natural light) control (Fig. 2). The changes of photosynthetic rate of two parents, 3037, Xiandans and Mars, after low light acclimation were significantly different from those of the control. After low light acclimation, the recovery of photosynthetic rate is not as good as that of undomesticated, and after given 30 minutes of darkness, more CO 2 is released. This indicated that the two hybrids F_1 and 02428 were less sensitive to light inhibition and had more adaptive ability to light than those from 3037, Xiandang and Mars. The hybrid F1 was derived from the parent 02428.