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建立测定消癌平片中通光散苷A的含量测定方法,为科学评价和有效控制消癌平片质量提供可靠的方法。采用高效液相色谱-蒸发光散射检测器(HPLC-ELSD)法,色谱柱为Agilent C18柱,流动相为乙腈-水(30∶70,V/V),流速为0.8 mL/min,进样量为10μL,柱温为35℃,ELSD检测器漂移管温度为106℃,载气流量为2.9 L/min。通光散苷A在3.57~9.57μg之间呈现良好的线性关系(r=0.999 4),平均加样回收率为100.5%,(RSD=1.3%,n=6)。建立了消癌平片中甾体皂苷类成分通光散苷A的HPLC-ELSD含量测定方法,该方法操作简便、准确、灵敏度高、重现性好,可作为消癌平片的质量控制方法。
To establish a method for the determination of the content of lucidum glycosides A in Xiaochang Tablets, which provides a reliable method for the scientific evaluation and effective control of the quality of the decoction plates. The column was an Agilent C18 column with acetonitrile-water (30:70, V / V) at a flow rate of 0.8 mL / min using high performance liquid chromatography with evaporative light scattering detector (HPLC-ELSD) The volume was 10 μL, the column temperature was 35 ° C, the ELSD detector drift tube temperature was 106 ° C and the carrier gas flow was 2.9 L / min. A good linear relationship was found between 3.57 ~ 9.57μg (r = 0.999 4). The average recovery was 100.5% (RSD = 1.3%, n = 6). A HPLC-ELSD method for the determination of the light-emitting glycosides of steroidal saponins in Xiao-Jia-Ping Tablets was established. The method is simple, accurate, sensitive, reproducible and can be used as a quality control method .