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利用生物发光（ＢＬ）技术进行高压灭菌效果监控，使传统的生物指示剂法实现了快速检测。当灭菌不彻底（只要有１×１０２ＣＦＵ／ｍｌ活菌体存在时）经５小时孵育即可观察到释放ＡＴＰ细菌数的增加。使ＢＬ发光峰值（ＣＰＳ）明显增高。用氯仿法释放指示菌ＡＴＰ进行ＢＬ测定，本底值重复性好，峰值＜１ＣＰＳ，按细菌分裂繁殖的生理特性，提出以孵育５小时后，ＢＬ发光峰值＞２ＣＰＳ判定为灭菌不彻底，与人工植入活菌法的实验结果一致。本法的应用对促进医院感染控制工作具有实用价值。
The use of bioluminescence (BL) technology to monitor the effects of autoclaving has enabled the rapid detection of traditional biological indicator methods. When the sterilization is not complete (as long as there is 1 × 102CFU / ml viable cells) incubated for 5 hours to observe the increase in the release of ATP bacteria. The BL luminescence peak (CPS) was significantly increased. Chlorophyll release of indicator bacteria ATP for BL determination, the background value of good reproducibility, the peak value <1CPS, according to the physiological characteristics of bacterial division and reproduction, proposed to incubate for 5 hours, BL luminescence peak> 2CPS judged as incomplete sterilization, and Artificial implantation of live bacteria method of the experimental results. The application of this law is of practical value in promoting hospital infection control.