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以对羟基联苯为内标设计了血浆中尼美舒利的高效液相色谱测定法。色谱柱为ＨｙｐｅｒｓｉｌＯＤＳ２２０ｃｍ×４．６ｍｍＩＤ５μｍ，流动相为甲醇－水－冰醋酸（６０４０１），流速１ｍｌ／ｍｉｎ，检测波长为２３０ｎｍ。血样经盐酸溶液酸化后以乙醚提取，回收率为８１．５３％～８９．１７％，血浆中尼美舒利的最低检出浓度为０．０２μｇ／ｍｌ，线性范围为０．１～２０μｇ／ｍｌ。１０名志愿者随机交叉ｐｏ单剂量２００ｍｇ两种国产尼美舒利制剂后，以本法测定其体内过程符合一室模型，统计学结果表明两种制剂吸收程度相近
Hydroxy-biphenyl as an internal standard design of plasma nimesulide high performance liquid chromatography. Column HypersilODS220cm × 4.6mmID5μm, the mobile phase of methanol - water - glacial acetic acid (60401), the flow rate of 1ml / min, the detection wavelength was 230nm. The blood samples were acidified with hydrochloric acid solution and extracted with diethyl ether. The recovery rates ranged from 81.53% to 89.17%. The lowest detectable concentration of nimesulide in plasma was 0.02μg / ml with a linear range of 0.1 ~ 20μg / ml. 10 volunteers randomly crossover po single dose of 200mg two domestic nimesulide formulations, measured by this method in vivo process in line with a one-compartment model, the statistical results show that the absorption of the two preparations were similar