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以载片上培养的大肠杆菌生物膜为对象,研究了氯对模拟管壁生物膜中大肠杆菌的灭活效果,同时考察氯氧化生物膜后水中生物可同化有机碳(AOC)、生物可利用磷(MAP)和细菌生长潜能(BRP)的变化情况.结果表明,氯可以有效灭活悬浮态的大肠杆菌,而对生物膜中的大肠杆菌的灭活效率远低于悬浮菌;在相同CT值下,较高浓度的氯对悬浮态和生物膜中大肠杆菌的灭活效果要高于低浓度的氯.氯对生物膜的氧化作用会使生物膜中物质溶出,增加了水中AOC和MAP浓度,如当氯的浓度为1.0 mg/L(CT值为100 mg.min/L)时,水中AOC由20.78μg/L增加到120.17μg/L,MAP含量由0.11μg/L增加到0.17μg/L;氯的氧化作用会增加水的细菌生长潜能(BRP),BRP随着CT值的增加而增加,如当氯投量为1.0 mg/L(CT值为100 mg.min/L)时,BRP可达到1.10×107CFU/mL.
The biofilms of Escherichia coli cultivated on slides were used to study the inactivation effect of chlorine on Escherichia coli in mimic wall biofilms. At the same time, the content of bio-assimilable organic carbon (AOC), bioavailable phosphorus (MAP) and bacterial growth potential (BRP) .Results showed that chlorine could effectively inactivate suspended E.coli, while the inactivation efficiency of E.coli in the biofilm was much lower than that of the suspension bacteria. In the same CT value , The higher concentration of chlorine inactivation of Escherichia coli in suspension and biofilm is higher than the low concentration of chlorine.Chloride oxidation of the biofilm will dissolve the material in the biofilm and increase the concentration of AOC and MAP in water , For example, when the concentration of chlorine is 1.0 mg / L (CT value is 100 mg.min / L), the AOC in water increases from 20.78μg / L to 120.17μg / L and the MAP content increases from 0.11μg / L to 0.17μg / L; the oxidation of chlorine increased the bacterial growth potential (BRP) of water, BRP increased with the increase of CT value. For example, when the chlorine dose was 1.0 mg / L (CT value was 100 mg.min / L) BRP up to 1.10 × 107CFU / mL.