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探讨丹参骨宝对维甲酸模型大鼠股骨生物力学及骨密度的影响。4月龄未交配的SPF级雌性SD大鼠40只,随机分成5组(每组8只):正常对照(CON)组、维甲酸模型(RA)组、丹参骨宝高剂量(DSH)组、丹参骨宝中剂量(DSM)组、丹参骨宝低剂量(DSL)组。CON组给予溶剂对照溶液,其它4组上午先用维甲酸70 mg.kg-1.d-1造模,然后同时下午给予不同药物预防,RA组给予溶剂对照溶液,DSH组、DSM组和DSL组分别给予含丹参素10、5和2.5 mg.kg-1.d-1的丹参骨宝。大鼠每次均按5 ml.kg-1灌胃给药,连续给药28 d。所有大鼠在处死前的第13、14 d和第3、4 d分别皮下注射盐酸四环素30 mg.kg-1和钙黄绿素5 mg.kg-1进行荧光标记。大鼠于实验结束时处死取材,取右侧股骨进行骨物理指标、骨密度和骨生物力学参数的测量。结果发现与CON组相比,RA组大鼠股骨物理指标(横断面的外径、湿重和干重)降低(P<0.05),股骨中点和远心端的骨密度均下降(P<0.05),股骨生物力学指标(最大载荷、弹性载荷、断裂应变、刚性系数、弯曲能量)均下降(P<0.05)。与RA组比较,DSH组和DSM组大鼠的股骨远心端的骨密度均增加(P<0.05),但其它指标无明显变化。本研究表明,维甲酸致大鼠股骨物理指标、骨密度和生物力学参数均下降,丹参骨宝可对抗维甲酸致大鼠股骨骨密度的降低,但对物理指标和生物力学参数无明显影响。
To investigate the effect of Danshen Guobao on femoral biomechanics and bone mineral density in rat model of retinoic acid. Forty SPF female SD rats, aged 4 months, were randomly divided into 5 groups (8 in each group): normal control (CON) group, RA model group, DSS group , Danshen Guobao medium dose (DSM) group, Danshen Guobao low dose (DSL) group. CON group were given solvent control solution, the other four groups in the morning with retinoic acid 70 mg.kg-1.d-1 modeling, and at the same time give different drug prevention, RA group given solvent control solution, DSH group, DSM group and DSL Group were given Danshensu 10,5 and 2.5 mg.kg-1.d-1 Danshen Guobao. Rats were administered 5 ml.kg-1 intragastrically every day for 28 days. All rats were subcutaneously injected with tetracycline hydrochloride (30 mg · kg-1) and calcein (5 mg · kg-1) on the 13th, 14th and 3rd and 3rd day before sacrifice. The rats were sacrificed at the end of the experiment, and the right femur was measured for bone physical parameters, bone mineral density and bone biomechanical parameters. The results showed that compared with the CON group, the femoral physical indexes (outer diameter, wet weight and dry weight) of the RA group decreased (P <0.05), and the BMD of the mid and distal femur decreased ), Femoral biomechanical parameters (maximum load, elastic load, strain at break, stiffness coefficient, bending energy) decreased (P <0.05). Compared with RA group, the BMD of distal femur in DSH group and DSM group increased (P <0.05), but there was no significant change in other indexes. The present study shows that the physical indexes of femur, bone mineral density and biomechanical parameters of rats induced by retinoic acid are decreased. Salvia miltiorrhiza can counteract the reduction of femoral BMD of rats induced by retinoic acid, but has no obvious effect on physical indexes and biomechanical parameters.