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从患病的杜氏肾脏分离出Y 1菌。人工感染试验 ,当注射9.0×107 CFU/尾时 ,Y 1菌可致试验鱼100%死亡 ;注射9.0×106CFU/尾时 ,死亡率为50% ,症状与自然发病相似 ,结果表明该分离菌为致病菌。Y 1菌为 :革兰氏阴性 ,有动力 ,杆状 ,极生单鞭毛 ;发酵葡萄糖 ,还原硝酸盐 ,氧化酶、触酶阳性 ,赖氨酸脱羧酶、鸟氨酸脱羧酶、精氨酸双水解酶阴性。根据形态及生理生化特性 ,Y 1菌应为弧菌Vibriosp.。药敏试验结果表明 ,该菌对氨基糖甙类、呋喃类、
Y1 bacteria were isolated from the diseased Duchenne kidneys. In the artificial infection test, Y 1 bacteria could cause 100% death of the test fish when the injection was 9.0 × 107 CFU / tail; the mortality rate was 50% when the injection was 9.0 × 106 CFU / tail and the symptom was similar to the natural disease. The results showed that the isolates For pathogenic bacteria. Y 1 bacteria are: Gram-negative, motile, rod-shaped, polar flagella; fermenting glucose, reducing nitrate, oxidase, tentase positive, lysine decarboxylase, ornithine decarboxylase, arginine Double hydrolase negative. According to the morphological and physiological characteristics, Y1 should be Vibrios. Susceptibility test results show that the bacteria on the aminoglycosides, furans,