论文部分内容阅读
目的观察拉莫三嗪对脑缺血再灌注大鼠血清神经元特异性烯醇化酶(NSE)与肿瘤坏死因子-α(TNF-α)的影响。方法线栓法闭塞大脑中动脉制作脑缺血再灌注大鼠模型;采用ELISA双抗体夹心法测定血清TNF-α水平,应用酶标免疫法检测血清NSE含量。结果缺血再灌注损伤组血清NSE与TNF-α水平较正常对照组和假手术对照组显著升高(P<0.01),拉莫三嗪20mg/kg治疗后血清NSE与TNF-α水平较缺血再灌注损伤组显著降低(P<0.01);术前1h给药组血清NSE与TNF-α水平较术后3h给药组与6h给药组显著降低,但术后3h给药组、6h给药组血清NSE与TNF-α水平的差别均无统计学意义,并且拉莫三嗪能改善大鼠神经功能评分,降低脑水肿。结论拉莫三嗪能减少NSE释放,并抑制促炎细胞因子TNF-α产生,减轻脑水肿,保护神经元,减轻脑缺血性损伤。
Objective To observe the effect of lamotrigine on serum neuron-specific enolase (NSE) and tumor necrosis factor-α (TNF-α) in rats with cerebral ischemia-reperfusion. Methods The rat model of cerebral ischemia-reperfusion was established by blocking occlusion of the middle cerebral artery with thread occlusion method. The level of serum TNF-α was determined by enzyme-linked immunosorbent assay (ELISA). Enzyme-linked immunosorbent assay was used to detect the serum NSE level. Results Serum levels of NSE and TNF-α in ischemia-reperfusion injury group were significantly higher than those in normal control group and sham operation control group (P <0.01). Serum levels of NSE and TNF-α in 20 mg / kg lamotrigine group were significantly lower than those in control group Serum levels of NSE and TNF-α in the 1 h preoperative group were significantly lower than those in the 3 h and 6 h postoperatively groups, but the levels of NSE and TNF-α in the 1 h preoperative group were significantly lower than those in the 6 h and 6 h postoperatively There was no significant difference in serum NSE and TNF-α levels between the two groups, and lamotrigine could improve the neurological function scores and reduce the cerebral edema in rats. Conclusions Lamotrigine can reduce the release of NSE, inhibit the production of pro-inflammatory cytokine TNF-α, relieve cerebral edema, protect neurons and alleviate cerebral ischemic injury.