论文部分内容阅读
对影响白菜游离小孢子培养的关键因素进行分析研究。单因素研究结果表明:54个基因型之间的小孢子胚诱导率差异显著;对供体材料低温处理,在0~5d范围内小孢子胚诱导率差异不大,超过5d则明显降低;高温诱导在12~60h范围内差异不大,超过此范围则小孢子胚诱导率明显降低;NLN培养基中添加生长素(NAA)和细胞分裂素(6-BA)对小孢子胚诱导率影响不大,添加的浓度过大时小孢子胚诱导率反而降低;活性炭的有无和浓度大小对小孢子胚诱导率影响极大。通过基因型、生长素、细胞分裂素、活性炭4因素分析表明:基因型之间差异显著,活性炭不同浓度之间差异显著;基因型与活性炭不同浓度之间的互作差异显著;其它的5个一级互作、4个二级互作和1个三级互作差异均不显著。
The key factors affecting cabbage free microspore culture were analyzed. The results of single factor study showed that there were significant differences in the induction rate of microspore embryos among 54 genotypes. The induction rate of microspore embryos in 0 ~ 5d range was not significant when the donor material was treated at low temperature, but significantly decreased after more than 5 days. The induction rate of microspore embryos was not significantly different within 12-60h, while the induction rate of microspore embryos beyond this range was significantly reduced. The addition of auxin (NAA) and cytokinin (6-BA) to NLN medium had no effect on the induction rate of microspore embryos The inducing rate of microspore embryos decreased when the concentration was too large. The presence or absence of activated carbon and the concentration of microspore embryos had a great effect on the induction rate of microspore embryos. The results of genotype, auxin, cytokinin and activated carbon showed that there was significant difference among genotypes, significant difference between different concentration of activated carbon and significant difference between different genotypes and activated carbons. The other 5 There was no significant difference in first level interaction, four second level interaction and one third level interaction.