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Objective: To determine the antioxidant and hepatoprotective potential of methanol extract of rhizome of Curcuma angustifolia (MECA) against carbon tetrachloride (CCl4)-induced hepatic damage in vitro and in vivo. Methods: DPPH, ABTS and reducing power assays were performed to estimate the antioxidant effect of MECA. In vitro cytotoxicity of MECA against HepG2 cells was evaluated, whereas serum biochemical parameters and levels of antioxidative enzymes were measured in vivo and in vitro. Additionally, histopathological studies were estimated in order to investigate the hepatoprotective efficacy of MECA. Furthermore, GC-MS analysis of the extract was performed to identify the chemical components. Results: MECA exhibited strong antioxidant activity and attenuated CCl4-induced decrease in the viability of HepG2 cells. Additionally, MECA significantly restored the ALT, AST, ALP, TP and albumin level in comparison with the CCl4 group. After pre-treatment with MECA, effects of SOD, CAT and GSH were increased as well as lipid peroxidation amount decreased on CCl4-induced hepatotoxicity in in vitro and in vivo model. Furthermore, histopathological observation confirmed that MECA reduced liver injury induced by CCl4 in rats. GC-MS analysis confirmed the presence of bioactive constituents such asα-tocopherol (12.27％), phytol (7.61％), squalene (3.71％), β-sitosterol (2.19％), eugenol (2.59％), curcumenol (1.20％), β-elemene (1.00％) and eucalyptol (0.89％). Conclusions: MECA contains antioxidant and hepatoprotective constituents such asα-tocopherol, phytol, squalene and eugenol and exerts hepatoprotective effect both in vitro and in vivo.