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三、不同繁殖方式对菌株类型的改变采用菌丝体转管(MI),组织分离(TC)以及孢子分离(SI)三种常用公式。(1)菌丝体转管不能改变稳定气生型或匍匐型菌株的类型,而对于混合型,定向菌丝体转管可以改变原始菌种的性状并有向气生与匍匐两个方向发展的趋势。如同原始菌种Y_3定向转管后,栽培产量Y_3F=10g。Y_3V=1319.67g、Y_3A=1146.33g。(2)组织分离也很少能改变菌株的性状,不过气生型菌株的子实体经组织分离后,在PDA上往往出现棕色。皱折匍匐状菌落,与匍匐型的白色粉状或束状菌落不同,这种棕色皱折菌落转管数次后气生性开始回复。栽培试验显示MI与TC差异不大,而同工酶测定两者间无差异。(3)稳定气生型与匍匐型菌株各一个,经
Third, different breeding methods to change the type of strains using mycelium tube (MI), tissue separation (TC) and spore separation (SI) three commonly used formula. (1) Mycelium tube can not change the type of stable air-born or creeping strains, while for mixed-type, directional mycelium tube can change the characteristics of the original species and develop in both aerial and creeping directions the trend of. As the original strain Y_3 directional tube, the cultivation yield Y_3F = 10g. Y_3V = 1319.67g, Y_3A = 1146.33g. (2) Tissue sequestration also seldom changed the traits of the strains, but the fruiting bodies of the avirulent strains often appeared brown on the PDA after tissue separation. Ruffled prostrate colonies, and creeping white powder or bundle-like colonies are different, this brown folds colonial tube several times after the start of the recovery of gas. Cultivation experiments showed little difference between MI and TC, while no difference between the isozyme assay. (3) Stable air-born and creeping strains each one, after