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从单纯疱疹病毒Ⅰ型ＨＳＶ－１基因组中分离出其包装信号序列，与含ＨＳＶ－１复制起点及ＩＥ－６８基因启动子的ＤＮＡ序列、β半乳糖苷酶基因和大肠杆菌质粒骨架，构建了一种ＨＳＶ－１扩增子质粒载体ｐＨＳＬ，其中ＬａｃＺ基因置于ＩＥ－６８基因启动子控制下。将此质粒转染已感染了ＨＳＶ－１温度敏感株ｔｓＫ株的Ｖｅｒｏ细胞，ｐＨＳＬ可在ＨＳＶ－１ｔｓＫ的辅助下包装成假病毒颗粒，这样就可得到同时含有假病毒和ＨＳＶ－１ｔｓＫ的混合毒株ｄｖＨＳＬ。将此混合毒株感染传代细胞和神经细胞，可在其中表达ＬａｃＺ基因，而在生理温度（３７℃）下，混合毒株的复制受到抑制。提示这种缺陷型疱疹病毒载体有用于向神经系统基因转移的可能性。
The packaging signal sequence was isolated from the herpes simplex virus type 1 HSV-1 genome, and the DNA sequence encoding the HSV-1 origin of replication and the promoter of IE-68 gene, the β-galactosidase gene and the plasmid backbone of Escherichia coli An HSV-1 amplicon plasmid pHSL, in which the LacZ gene is placed under the control of the IE-68 gene promoter. This plasmid was transfected into Vero cells that had been infected with the HSV-1 temperature-sensitive strain tsK and pHSL was packaged as a pseudovirion with the aid of HSV-1tsK to obtain a mixed virus containing both pseudoviruses and HSV-1tsK Strains dvHSL. The mixed strain was infected in the passage cells and nerve cells, in which LacZ gene expression, and at physiological temperature (37 ℃), the replication of mixed strains was inhibited. Suggesting that this defective herpesvirus vector has the potential for gene transfer to the nervous system.